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Quantification of Manila clam ruditapes philippinarum (Adams & Reeve, 1850) larvae based on SYBR green real-time polymerase chain reaction

AuthorsQuinteiro, Javier; Pérez-Diéguez, L.; Sánchez, Ana Cristina ; Pérez Martín, Ricardo Isaac ; González Sotelo, Carmen ; Rey Méndez, Manuel
KeywordsManila clam
Ruditapes philippinarum
16S rRNA
Real-time PCR
SYBR Green
Larvae detection
Larvae quantification
Issue Date2011
PublisherNational Shellfisheries Association
CitationJournal of Shellfish Research 30(3): 791-796 (2011)
AbstractBiological and ecological research is seriously handicapped because of difficulties experienced in the reliable detection and quantification of bivalve larvae. This is a critical issue in the case of the Manila clam Ruditapes philippinarum (Adams & Reeve, 1850), a largely invasive and commercially relevant species, with important wild, cultured, and naturalized populations throughout the world. A SYBR Green real-time PCR assay, containing TPHI16S1F and TPHI16S2R primers (specific to R. philippinarum female mtDNA), was designed and tested to provide a rapid and high-throughput PCR-based method for larval quantification. Accurate estimations of larval numbers in spiked plankton samples point to the usefulness of this system. It can be used through a wide range of evaluated variable experimental conditions, such as the presence of closely related bivalve species, stationary-dependent plankton abundance, sampling volumes, and larval size.
Description6 páginas, 1 tabla, 5 figuras
Publisher version (URL)http://dx.doi.org/10.2983/035.030.0319
Appears in Collections:(IIM) Artículos
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