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Título

Fast monitoring of species-specific peptide biomarkers using high-intensity-focused-ultrasound-assisted tryptic digestion and selected MS/MS ion monitoring

AutorCarrera, Mónica ; Cañas, Benito; López-Ferrer, Daniel; Piñeiro, Carmen ; Vázquez, Jesús ; Gallardo, José Manuel
Palabras claveMass spectrometry
Biomaker
Selected MS/SM ion monitoring (SMIM)
High-intensity focused ultrasound (HIFU)
Proteomics
Parvalbumin
Fish
Food safety
Fecha de publicación2011
EditorAmerican Chemical Society
CitaciónAnalytical Chemistry - Columbus 83(14): 5688-5695 (2011)
ResumenA new strategy for the fast monitoring of peptide biomarkers is described. It is based on the use of accelerated in-solution trypsin digestions under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of several peptides by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The performance of the method was established for the unequivocal identification of all commercial fish species belonging to the Merlucciidae family. Using a particular combination of only eleven peptides, resulting from the HIFU-assisted tryptic digestion of the thermostable proteins parvalbumins (PRVBs), the workflow allowed the unequivocal identification of these closely related fish species in any seafood product, including processed and precooked products, in less than 2 h. The present strategy constitutes the fastest method for peptide biomarker monitoring. Its application for food quality control provides to the authorities an effective and rapid method of food authentication and traceability to guarantee the quality and safety to the consumers.
Descripción2 tablas, 3 figuras
Versión del editorhttp://dx.doi.org/10.1021/ac200890w
URIhttp://hdl.handle.net/10261/47506
DOI10.1021/ac200890w
ISSN0003-2700
E-ISSN1520-6882
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