English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/47371
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Natural abundance of 15N and 13C in fish tissues and the use of stable isotopes as dietary protein tracers in rainbow trout and gilthead sea bream

AuthorsBeltrán, Make; Fernández-Borràs, Jaume; Médale, Françoise; Pérez-Sánchez, Jaume ; Kaushik, Sadasivam; Blasco, Josefina
Rainbow trout
Sea bream
Issue DateFeb-2009
PublisherBlackwell Publishing
CitationAquaculture Nutrition 15(1): 9-18 (2009)
AbstractFor developing efficient diets, two sets of experiments examined whether the use and allocation of dietary protein can be traced by labelling with stable isotopes (15N and 13C) in two culture fish (Oncorhynchus mykiss and Sparus aurata). In the first experiment, natural abundance and tissue distribution of these isotopes were determined, by measuring the δ13C and δ15N values by isotopic ratio mass spectrometry, in fingerlings (14-17 g) adapted to diets differing in the percentage of fish meal replacement by plant protein sources. For both species, δ15N and δ13C were greater in tissues with higher protein and lower lipid content. Delta 15N of diets and tissues decreased as replacement increased, suggesting δ15N can be used as a marker for dietary protein origin. The 15N fractionation (δ15N fish - δ15N diet) differed between groups, and could thus be used to indicate protein catabolism. In the second experiment, fish (75-90 g) of each species ingested a diet enriched with 15N-protein (10 g kg-1 diet) and 13C-protein (30 g kg-1 diet). These proportions were suitable for determining that the delta values of tissue components were high enough above natural levels to allow protein allocation to be traced at 11 and 24 h after feeding, and revealed clear metabolic differences between species.
Description10 p., 5 tables, 7 figures and bibliography
Publisher version (URL)http://dx.doi.org/10.1111/j.1365-2095.2008.00563.x
Appears in Collections:(IATS) Artículos
Files in This Item:
File Description SizeFormat 
restringido.pdf21,67 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.