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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/47318
Título

Characterization of Human Thioredoxin-like 2. A novel microtubule-binding thioredoxin expressed predominantly in the cilia of lung airway epithelium and spermatid manchette and axoneme

AutorSadek, Christine M.; Jiménez, Alberto ; Damdimopoulos, Anastasios E.; Kieselbach, Thomas; Nord, Magnus; Gustafsson, Jan-Åke; Spyrou, Giannis; Davis, Elaine C.; Oko, Richard; Hoorn, Frans A. van der; Miranda-Vizuete, Antonio
Palabras claveThioredoxin
NDP kinase
Cilia
Flagella
Spermatozoa
Fecha de publicación4-feb-2003
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónJournal of Biological Chemistry 278(15): 13133-13142 (2003)
ResumenWe describe here the cloning and characterization of a novel member of the thioredoxin family, thioredoxin-like protein 2 (Txl-2). The Txl-2 open reading frame codes for a protein of 330 amino acids consisting of two distinct domains: an N-terminal domain typical of thioredoxins and a C-terminal domain belonging to the nucleoside-diphosphate kinase family, separated by a small interface domain. The Txl-2 gene spans approximately 28 kb, is organized into 11 exons, and maps at locus 3q22.3-q23. A splicing variant lacking exon 5 (Delta 5Txl-2) has also been isolated. By quantitative real time PCR we demonstrate that Txl-2 mRNA is ubiquitously expressed, with testis and lung having the highest levels of expression. Unexpectedly, light and electron microscopy analyses show that the protein is associated with microtubular structures such as lung airway epithelium cilia and the manchette and axoneme of spermatids. Using in vitro translated proteins, we demonstrate that full-length Txl-2 weakly associates with microtubules. In contrast, Delta 5Txl-2 specifically binds with very high affinity brain microtubule preparations containing microtubule-binding proteins. Importantly, Delta 5Txl-2 also binds to pure microtubules, proving that it possesses intrinsic microtubule binding capability. Taken together, Delta 5Txl-2 is the first thioredoxin reported to bind microtubules and might therefore be a novel regulator of microtubule physiology.
Descripción48 páginas, 11 figuras, 1 tabla.
Versión del editorhttp://dx.doi.org/10.1074/jbc.M300369200
URIhttp://hdl.handle.net/10261/47318
DOI10.1074/jbc.M300369200
ISSN0021-9258
E-ISSN1083-351X
ReferenciasPMID: 12569107
PMC3158135
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