English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/47221
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Methods for Growing and Titrating African Swine Fever Virus Field and Laboratory Virus Samples

AutorCarrascosa, Ángel L.; Bustos, Maria José; León, Patricia de
Palabras claveAfrican swine fever virus (ASFV)
Susceptibility
Fecha de publicación1-dic-2011
EditorJohn Wiley & Sons
CitaciónCurrent Protocols in Cell Biology 53:26.14.1-26.14.25 (20011)
ResumenGrowing African swine fever virus (ASFV) isolates obtained mainly from the field, but also engineered in the laboratory, is a critical step for diagnosis, titration, or virus infection studies. This unit describes a set of methods and protocols to produce and titrate any ASFV strain in cell cultures. The procedures include (1) basic techniques to prepare virus-sensitive target cells; (2) strategies for growth, concentration, and purification of virus stocks; and (3) the semi-quantitative (end dilution) and quantitative (plaque) assays for the determination of viral titers, and the use of different ASFV-sensitive cells as targets for virus production and titration.
Versión del editorhttp://dx.doi.org/10.1002/0471143030.cb2614s53
URIhttp://hdl.handle.net/10261/47221
DOI10.1002/0471143030.cb2614s53
ISSN1934-2500
E-ISSN1934-2616
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.