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Feasibility and anatomical development of an in vitro olive cleft-graft

AutorTroncoso de Arce, Antonio ; Liñán Benjumea, Juana ; Cantos, Manuel ; Acebedo, M. M.; Rapoport, Hava F.
Palabras claveIn vitro culture
Plant growth
Fecha de publicación1999
CitaciónJournal of Horticultural Science and Biotechnology 74(5): 584-587 (1999)
ResumenThe feasibility and anatomical development of an in vitro olive cleft-graft method were studied. Grafting survival after 60 d in vitro was 85% which then dropped slightly to 67% after hardening. Three days after grafting, callus formation was observed along the cut surfaces of the rootstock and scion, after 6 d the first healing cellular unions were observed, and by 12 d after grafting a strong union developed. The first cellular differentiation to form vascular tissues was observed 12 d after grafting and continued rapidly until a total connection was reached 10 d later. Thus a complete graft union of the in vitro olive cleft-grafts was achieved in three weeks, and vigorous plants were established after 60 d of in vitro culture followed by 10 d of hardening. The in vitro olive cleft graft is suggested as an effective and useful method for germplasm multiplication.
Descripción4 pages, 4 figures, 22 references.
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