English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/47120
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Induced biosynthesis of liver glucokinase

AutorSols, Alberto; Salas, Margarita ; Viñuela, Eladio
Palabras claveGlucokinase
Messenger RNA
Hexosemonophosphate
Liver
Fecha de publicación1964
EditorPergamon Press
CitaciónAdvances in Enzyme Regulation, 2: 177-188 (1964)
ResumenA glucokinase qualitatively and quantitatively adequate as the first and limiting step in the synthesis of glycogen from glucose in liver has recently been identified in normal fed rats. In contrast with hexokinase, that also occurs in liver, glucokinase activity disappears in fasted and alloxandiabetic animals. The high Km, glucose-6-P-insensitive glucokinase, does not occur in muscle, nor in any of several other tissues examined. This and other indirect evidence suggest that the glucokinase is a unique enzyme of the parenchymal cells of the liver, and that the hexokinase measured in liver homogenates is present in the non-parenchymal cells. Glucokinase reappears in diabetic and fasted animals within a few hours after administration of insulin or refeeding respectively. Glucose administration alone is also effective in provoking the reappearance of glucokinase activity in previously fasted rats. Liver hexokinase remains essentially unaltered in these conditions. Inhibition of the reappearance of glucokinase by ethionine and p-fluorophenylalanine, largely counteracted by methionine and phenylalanine respectively, suggests that actual synthesis of the enzyme is involved. A similar effect of actinomycin supports this conclusion while it further suggests that formation of messenger RNA is involved. These results suggest that liver glucokinase is an inducible enzyme whose synthesis is induced directly or indirectly, by insulin. The differences in the hexosemonophosphate pathway between liver and muscle, their metabolic regulation, and the relationship with other pathways is discussed.
Versión del editorhttp://dx.doi.org/10.1016/S0065-2571(64)80012-1
URIhttp://hdl.handle.net/10261/47120
DOI10.1016/S0065-2571(64)80012-1
ISSN0065-2571
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Embargo.pdf21,67 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.