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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/46900
Título

Pheromone synthesis in a biomicroreactor coated with anti-adsorption polyelectrolyte multilayer

AutorDimov, Nikolay; Muñoz, Lourdes; Carot-Sans, Gerard; Verhoeven, Michel L. P. M.; Bula, Wojciech P.; Kocer, Gülistan; Guerrero, Ángel; Gardeniers, Han J. G. E.
Palabras claveAdsorption
Atomic force microscopy
Biochemistry
Biological techniques
BioMEMS
Bioreactors
Chromatography
Electrophoretic coatings
Ellipsometry
Enzymes
Mass spectroscopic chemical analysis
Microfluidics
Microreactors
Molecular biophysics
Multilayers
Polymer electrolytes
Polymer films
Zoology
Fecha de publicación2011
EditorAmerican Institute of Physics
CitaciónBiomicrofluidics
ResumenTo prepare a biosynthetic module in an infochemical communication project, we designed a silicon/glass microreactor with anti-adsorption polyelectrolyte multilayer coating and immobilized alcohol acetyl transferase (atf), one of the key biosynthetic enzymes of the pheromone of Spodoptera littoralis, on agarose beads inside. The system reproduces the last step of the biosynthesis in which the precursor diene alcohol (Z,E)-9,11-tetradecadienol is transformed into the major component (Z,E)-9,11-tetradecadienyl acetate. The scope of this study was to analyze and implement a multilayer, anti-adsorption coating based on layer-by-layer deposition of polyethylenimine/dextransulfate sodium salt (PEI/DSS). The multilayers were composed of two PEI with molecular weights 750 and 1.2 kDa at pH 9.2 or 6.0. Growth, morphology, and stability of the layers were analyzed by ellipsometry and atomic force microscopy (AFM). The anti-adsorption functionality of the multilayer inside the microreactor was validated. The activity of His6-(atf) was measured by gas chromatography coupled to mass spectrometer (GC-MS).
Versión del editorhttp://dx.doi.org/10.1063/1.3608138
URIhttp://hdl.handle.net/10261/46900
DOI10.1063/1.3608138
E-ISSN1932-1058
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