English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/46832
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Molecular characterisation of the small-eukaryote community in a tropical Great Lake (Lake Tanganyika, East Africa)

AuthorsTarbe, Anne-Laure; Stenuite, Stephane; Balagué, Vanessa ; Sinyinza, Danny; Descy, Jean-Pierre; Massana, Ramon
KeywordsSmall-eukaryote community
Tropical lake
18S rRNA
Issue DateJan-2011
PublisherInter Research
CitationAquatic Microbial Ecology 62(2): 177-190 (2011)
AbstractIn aquatic environments, small eukaryotes (mainly algae and protozoa of 1 to 5 µm in size) are a key link in the carbon transfer to higher trophic levels, e.g. through primary production and grazing of picoplankton. However, the diversity of these microorganisms remains poorly investigated in freshwater habitats, and is still unknown in tropical aquatic systems. In this study, we investigated the small-eukaryote diversity in the oligotrophic Lake Tanganyika, one of the African Great Lakes, at different depths in the water column using denaturing gradient gel electrophoresis (DGGE) and gene clone libraries based on 18S rRNA genes. Each sample produced complex DGGE fingerprints clearly discriminating the epilimnion from the metalimnion. Analysis, using genetic libraries, confirmed the high level of small-eukaryote diversity in Lake Tanganyika. Organisms from 5 taxonomic groups (Stramenopiles, Alveolata, Cryptophyta, Kinetoplastea and Choanoflagellida) were dominant among the species detected. Some sequences were nearly identical to those recovered in temperate freshwaters in North America and Europe, suggesting a high dispersal ability in some small-eukaryote lineages. However, 49% of sequences were <95% similar to any sequence in GenBank. This may result from undersampling of freshwater systems, but also raises the possibility that perennially warm tropical waters harbour particular assemblages of planktonic small eukaryotes
Description14 pages, 7 figures, 3 tables
Publisher version (URL)https://doi.org/10.3354/ame01465
URIhttp://hdl.handle.net/10261/46832
DOI10.3354/ame01465
ISSN0948-3055
E-ISSN1616-1564
Appears in Collections:(ICM) Artículos
Files in This Item:
File Description SizeFormat 
Tarbe_et_al_2011.pdf2,68 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.