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Synthesis and Properties of Oligonucleotides Carrying Isoquinoline Imidazo[1,2-a]azine Fluorescent Units

AuthorsPérez-Rentero, Sonia; Kielland, Nicola; Terrazas, Montserrat ; Lavilla, Rodolfo; Eritja Casadellà, Ramón
Issue Date5-Aug-2010
PublisherAmerican Chemical Society
CitationBioconjugate Chemistry 21(9): 1622–1628 (2010)
AbstractOligonucleotides carrying novel fluorescent compounds with a dipolar isoquinoline imidazo[1,2-a]azine core were prepared. Analysis of the melting curves demonstrates that DNA duplexes carrying these fluorescent labels at their ends have a slight increase in DNA duplex stability. The UV absorption and fluorescent properties of the oligonucleotide conjugates were analyzed. The fluorescent label is sensitive to duplex formation, as cooperative melting curves are also observed at 366 nm and fluorescence has a large increase upon denaturation. Cell uptake studies allow observation of these fluorescently labeled oligonucleotides internalized into HeLa cells.
Publisher version (URL)http://dx.doi.org/10.1021/bc1000966
Appears in Collections:(IQAC) Artículos
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