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Title

Hydroxylation of naphthalene by aromatic peroxygenase from Agrocybe aegerita proceeds via oxygen transfer from H(2)O (2) and intermediary epoxidation

AuthorsKluge, Martín; Ullrich, René; Dolge, Christoph; Scheibner, Katrin; Hofrichter, Martin
KeywordsPeroxidase
Oxygenation
Hydroxylation
P450
Naphthol
Issue Date2009
PublisherSpringer
CitationApplied Microbiology and Biotechnology 81(6): 1071-1076(2009)
AbstractA-grocybe aegerita peroxidase/peroxygenase (AaP) is an extracellular fungal biocatalyst that selectively hydroxylates the aromatic ring of naphthalene. Under alkaline conditions, the reaction proceeds via the formation of an intermediary product with a molecular mass of 144 and a characteristic UV absorption spectrum (A (max) 210, 267, and 303 nm). The compound was semistable at pH 9 but spontaneously hydrolyzed under acidic conditions (pH <7) into 1-naphthol as major product and traces of 2-naphthol. Based on these findings and literature data, we propose naphthalene 1,2-oxide as the primary product of AaP-catalyzed oxygenation of naphthalene. Using (18)O-labeled hydrogen peroxide, the origin of the oxygen atom transferred to naphthalene was proved to be the peroxide that acts both as oxidant (primary electron acceptor) and oxygen source.
Description6 páginas, 3 figuras -- PAGS nros. 1071-1076
Publisher version (URL)http://dx.doi.org/10.1007/s00253-008-1704-y
URIhttp://hdl.handle.net/10261/46712
DOI10.1007/s00253-008-1704-y
ISSN0175-7598
E-ISSN1432-0614
Appears in Collections:(CIB) Artículos
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