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Hight Throughput, Parallel Imaging and Biomarker Quantification of Human Spermatozoa by ImageStream Flow Cytometry

AuthorsBuckman, Clayton; George, Thaddeus C.; Friend, Sherree; Sutovsky, Miriam; Miranda-Vizuete, Antonio ; Ozanon, Christophe; Morrissey, Phil; Sutovsky, Peter
KeywordsFlow cytometry
Male infertility
Semen analysis
Issue DateDec-2009
PublisherInforma Healthcare
CitationSystems Biology in Reproductive Medicine 55(5-6): 244-251 (2009)
AbstractSpermatid specific thioredoxin-3 protein (SPTRX-3) accumulates in the superfluous cytoplasm of defective human spermatozoa. Novel ImageStream technology combining flow cytometry with cell imaging was used for parallel quantification and visualization of SPTRX-3 protein in defective spermatozoa of five men from infertile couples. The majority of the SPTRX-3 containing cells were overwhelmingly spermatozoa with a variety of morphological defects, detectable in the ImageStream recorded images. Quantitative parameters of relative SPTRX-3 induced fluorescence measured by ImageStream correlated closely with conventional flow cytometric measurements of the same sample set and reflected the results of clinical semen evaluation. Image Stream quantification of SPTRX-3 combines and surpasses the informative value of both conventional flow cytometry and light microscopic semen evaluation. The observed patterns of the retention of SPTRX-3 in the sperm samples from infertility patients support the view that SPTRX3 is a biomarker of male infertility.
Description8 páginas, 2 figuras, 1 tabla.
Publisher version (URL)http://dx.doi.org/10.3109/19396360903056224
Appears in Collections:(CABD) Artículos
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Table 1_FINAL_FINAL copy.docTabla43 kBMicrosoft WordView/Open
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