Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/46669
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Rivas, Blanca de las | - |
dc.contributor.author | Rodríguez, Héctor | - |
dc.contributor.author | Carrascosa, Alfonso V. | - |
dc.contributor.author | Muñoz, Rosario | - |
dc.date.accessioned | 2012-03-06T12:21:04Z | - |
dc.date.available | 2012-03-06T12:21:04Z | - |
dc.date.issued | 2007-01 | - |
dc.identifier.citation | Journal of Applied Microbiology 104(1): 194–203 (2007) | es_ES |
dc.identifier.issn | 1364-5072 | - |
dc.identifier.uri | http://hdl.handle.net/10261/46669 | - |
dc.description.abstract | Aims: Histamine intoxication is probably the best known toxicological problem of food-borne disease. A histamine-producing Staphylococcus capitis strain has been isolated from a cured meat product. The aim of this study was to gain deeper insights into the genetic determinants for histamine production in Staph. capitis. Methods and Results: The nucleotide sequence of a 6446-bp chromosomal DNA fragment containing the hdcA gene encoding histidine decarboxylase (HDC) has been determined in Staph. capitis IFIJ12. This DNA fragment contains five complete and two partial open reading frames. Putative functions have been assigned to gene products by sequence comparison with proteins included in the databases. The hdcA gene has been expressed in Escherichia coli resulting in HDC activity. The presence of a functional promoter (Phdc) located upstream of hdcA has been demonstrated. Insertion of the histamine biosynthetic locus in Staph. capitis seems to be associated with a noticeable genome reorganization. Conclusions: Among the staphylococcal species analysed in this study only Staph. capitis strains produce histamine. The hdcA gene cloned from Staph. capitis encodes a functional HDC that produce histamine from the amino acid histidine. Significance and Impact of the Study: The identification of the DNA region involved in histamine production in Staph. capitis will allow further work in order to avoid histamine production in foods. | es_ES |
dc.description.sponsorship | This work was supported by grants AGL2005-00470 (CICYT), FUN-C-FOOD Consolider 25506 (MEC), RM03- 002 (INIA) and S-0505 ⁄AGR⁄ 000153 (CAM). We thank A. Marcobal for help in the construction of the pAM28 plasmid. The technical assistance M.V. Santamaría is greatly appreciated. H. Rodríguez is a recipient of an I3P predoctoral fellowship from the CSIC. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | John Wiley & Sons | es_ES |
dc.relation.isversionof | Postprint | - |
dc.rights | openAccess | es_ES |
dc.subject | Biogenic amines | es_ES |
dc.subject | Coagulase-negative | es_ES |
dc.subject | staphylococci | es_ES |
dc.subject | histamine | es_ES |
dc.subject | histidine | es_ES |
dc.title | Molecular cloning and functional characterization of a histidine decarboxylase from Staphylococcus capitis | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1111/j.1365-2672.2007.03549.x | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1111/j.1365-2672.2007.03549.x | es_ES |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | - |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | - |
dc.contributor.funder | CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100007652 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (IFI) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
staphylococcus_capitis_Rivas.pdf | 281,74 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
28
checked on 10-abr-2024
WEB OF SCIENCETM
Citations
27
checked on 29-feb-2024
Page view(s)
293
checked on 18-abr-2024
Download(s)
311
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.