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Título

Molecular cloning and functional characterization of a histidine decarboxylase from Staphylococcus capitis

AutorRivas, Blanca de las ; Rodríguez, Héctor; Carrascosa, Alfonso V. ; Muñoz, Rosario
Palabras claveBiogenic amines
coagulase-negative
staphylococci
histamine
histidine
Fecha de publicaciónene-2007
EditorJohn Wiley & Sons
CitaciónJournal of Applied Microbiology 104(1): 194–203 (2007)
ResumenAims: Histamine intoxication is probably the best known toxicological problem of food-borne disease. A histamine-producing Staphylococcus capitis strain has been isolated from a cured meat product. The aim of this study was to gain deeper insights into the genetic determinants for histamine production in Staph. capitis. Methods and Results: The nucleotide sequence of a 6446-bp chromosomal DNA fragment containing the hdcA gene encoding histidine decarboxylase (HDC) has been determined in Staph. capitis IFIJ12. This DNA fragment contains five complete and two partial open reading frames. Putative functions have been assigned to gene products by sequence comparison with proteins included in the databases. The hdcA gene has been expressed in Escherichia coli resulting in HDC activity. The presence of a functional promoter (Phdc) located upstream of hdcA has been demonstrated. Insertion of the histamine biosynthetic locus in Staph. capitis seems to be associated with a noticeable genome reorganization. Conclusions: Among the staphylococcal species analysed in this study only Staph. capitis strains produce histamine. The hdcA gene cloned from Staph. capitis encodes a functional HDC that produce histamine from the amino acid histidine. Significance and Impact of the Study: The identification of the DNA region involved in histamine production in Staph. capitis will allow further work in order to avoid histamine production in foods.
Versión del editorhttp://dx.doi.org/10.1111/j.1365-2672.2007.03549.x
URIhttp://hdl.handle.net/10261/46669
DOI10.1111/j.1365-2672.2007.03549.x
ISSN1364-5072
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