Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/46520
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | The txl1+ gene from Schizosaccharomyces pombe encodes a new thioredoxin-like 1 protein that participates in the antioxidant defence against tert-butyl hydroperoxide |
Autor: | Jiménez, Alberto CSIC ORCID CVN; Mateos, Laura; Pedrajas, José R.; Miranda-Vizuete, Antonio CSIC ORCID; Revuelta Doval, José Luis CSIC ORCID CVN | Palabras clave: | Thioredoxin-like Antioxidants Tert-butyl hydroperoxide Schizosaccharomyces pombe |
Fecha de publicación: | 3-may-2007 | Editor: | John Wiley & Sons | Citación: | Yeast 24(6): 481-490 (2007) | Resumen: | Yeasts are equipped with several putative single-domain thioredoxins located in different subcellular compartments. However, additional proteins containing thioredoxin domains are also encoded by the yeast genomes as described for mammals and other eukaryotic organisms. We report here the characterization of the fission yeast orthologue thioredoxin-like 1 (txl1+), which has been previously identified in mammals. Similarly to the human protein, the fission yeast Txl1 is a two-domain protein comprising an N-terminal thioredoxin-like domain and a C-terminal domain of unknown function. Many other yeasts and fungi species contain homologues of txl1+; however, there is no evidence of txl1+ orthologues in either Saccharomyces cerevisiae or plants. Txl1 is found in both the nucleus and the cytoplasm of Schizosaccharomyces pombe cells and exhibits a strong reducing activity coupled to thioredoxin reductase. In humans, TXL1 expression is induced by glucose deprivation and overexpression of TXL1 confers resistance against this stress. In contrast, a Sz. pombe Δtxl1 mutant was not affected in the response against glucose starvation but the Δtxl1 mutant strain showed a clear hypersensitivity to alkyl hydroperoxide. The mRNA levels of txl1+ in a h20 strain did not change in response to any oxidative insult (hydrogen peroxide or alkyl hydroperoxide) and the overexpression of an integrated copy of the wild-type txl1+ gene did not confer a significant increased resistance against alkyl hydroperoxide. Overall, these results indicate that the Txl1 role in the cellular detoxification of alkyl hydroperoxide is exerted through a constitutive transcription of txl1+. | Descripción: | 25 páginas, 6 figuras, 1 tabla. Tabla suplementaria. | Versión del editor: | http://dx.doi.org/10.1002/yea.1483 | URI: | http://hdl.handle.net/10261/46520 | DOI: | 10.1002/yea.1483 | ISSN: | 0749-503X | E-ISSN: | 1097-0061 |
Aparece en las colecciones: | (CABD) Artículos (IMB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Txl-1_pombe_final.doc | Texto | 266,5 kB | Microsoft Word | Visualizar/Abrir |
Figures Txl-1.pdf | Figuras 1-6 | 4,23 MB | Adobe PDF | Visualizar/Abrir |
Supplemental Data Txl-1 Table 1.doc | Tabla suplementaria | 24 kB | Microsoft Word | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
13
checked on 13-abr-2024
WEB OF SCIENCETM
Citations
14
checked on 24-feb-2024
Page view(s)
380
checked on 22-abr-2024
Download(s)
456
checked on 22-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.