Desarrollo de un protocolo de PCR a tiempo real para la detección de betanodavirus pertenecientes a los genotipos SJNNV y RGNNV

Abstract

[EN] Viral Encephalopathy and Retinopathy (VER) is a fish disease caused by nodaviruses included in the Betanodavirus genus. This disease has been a major obtacle for the aquaculture industry since these viruses affect to more than 30 marine fish species, and its control is based on the viral detection in asymptomatic specimens. Present study describes a SYBR Green I Real-Time PCR procedure for VNNV detection from several organs of seabass, sole and gilt-head seabream, either diseased or asymptomatic specimens. The sensivity of this technique was 10-2 TCID50ml-1. The newly designed primers allow for the specific detection of nodavirus isolates belonging to the RGNNV and SJNNV genotypes.

[ES] La enfermedad de la Necrosis Nerviosa Vírica (NNV) ocasionada por un betanodavirus (familia Nodaviridae) ha sido considerada como una gran amenaza para la acuicultura, afectando a más de 30 especies marinas de peces, entre ellas, la dorada (Sparus aurata), la lubina (Dicentrarchus labrax) (Munday et al., 2002) y, más recientemente, el lenguado senegalés (Solea senegalensis) (Thiery et al., 2004). El objetivo de este estudio ha sido el desarrollo de un sistema de PCR a tiempo real relativa para detectar betanodavirus pertenecientes a los genotipos SJNNV y RGNNV.