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Título

Conditional UAS-targeted repression in Drosophila

AutorPascual Bravo, Alberto ; Huang, Kai-Lian; Preat, Thomas
Palabras claveDNA-binding proteins
Drosophila
Nuclear proteins
Repressor proteins
Saccharomyces cerevisiae proteins
Transcription factors
Fecha de publicación13-ene-2005
EditorOxford University Press
CitaciónNucleic Acids Research 33(1): e7 (2005)
ResumenThe Gal4–UAS enhancer trap system is useful for driving gene expression in various tissues. A new tool that extends Gal4 technology is described here. A fusion protein containing the Gal4 binding domain and the repression domain of the isolator suppressor of hairy wing was placed under the control of a heat shock-inducible promoter. The construct mediates the conditional repression of genes located downstream of a UAS sequence. The repressive effects of the chimeric protein on fasII gene expression were tested by western-blot analysis and in brain sections of adult Drosophila. Owing to the increasing number of Gal4 and UAS transgenic lines, this versatile system will facilitate the study of gene function.
Descripción4 páginas, 3 figuras.
Versión del editorhttp://dx.doi.org/10.1093/nar/gni006
URIhttp://hdl.handle.net/10261/46431
DOI10.1093/nar/gni006
ISSN1362-4962
E-ISSN0305-1048
ReferenciasPMID: 15653628
PMC546177
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