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Título

Molecular markers to increase corn earworm resistance in a maize population

AutorButrón Gómez, Ana María ; Li, R. G.; Guo, B. Z.; Widstrom, N. W.; Snook, M. E.; Cleveland, T. E.; Lynch, R. E.
Palabras claveMaysin
Flavonoid
RFLP markers
Zea mays
Helicoverpa Zea
Husk tightness
Apimaysin
3 '-methoxymaysin
Chlorogenic acid
Fecha de publicación2001
EditorConsiglio per la ricerca e la sperimentazione in agricoltura (Italia)
CitaciónMaydica 46: 117-124 (2001)
ResumenMaysin and related compounds, such as apimaysin, 3 ' -methoxymaysin, and chlorogenic acid, have been determined to be important maize (Zea mays L.) produced antibiotic compounds against corn earworm (Helicoverpa zea Boddie), but, to be effective under field conditions, silk antibiotics should be accompanied by good husk coverage. The objective of this work was to identify molecular markers associated with synthesis of maysin and related compounds in a maize Population and determine if the markers could be linked to the genes which affect husk tightness. A total of 113 probes were used to screen for RFLP polymorphisms and the 53 probes that were polymorphic between the parents were used as codominant markers to genotype 205 F-2 individuals. Silks and husks of F-2:3 families were evaluated. Two major QTL were identified for the synthesis of maysin and related compounds, the already known pl, on the short arm of chromosome 1, and a novel one on the interval csu1066-umcl76 on genomic region 2C-2L. A QTL for husk tightness was located near pl. The functional allele for pi and the favorable allele for husk tightness were in repulsion linkage. in a marker-assisted selection program for increasing resistance to corn earworm, markers for silk antibiotic synthesis should be accompanied by markers for husk tightness. Efforts Should be made to convert the RFLP-markers into PCR-based markers for user friendly application in marker-assisted breeding.
URIhttp://hdl.handle.net/10261/46197
ISSN0025-6153
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