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| Título: | Role of mitogen-activated protein kinase Sty1 in regulation of eukaryotic initiation factor 2α kinases in response to environmental stress in Schizosaccharomyces pombe |
Autor: | Berlanga, Juan José; Martín, Ruth; Moreno, Sergio CSIC ORCID; Haro, César de CSIC | Fecha de publicación: | ene-2010 | Editor: | American Society for Microbiology | Citación: | Eukaryotic Cell 9(1): 194-207 (2010) | Resumen: | The mitogen-activated protein kinase (MAPK) Sty1 is essential for the regulation of transcriptional responses that promote cell survival in response to different types of environmental stimuli in Schizosaccharomyces pombe. In fission yeast, three distinct eukaryotic initiation factor 2α (eIF2) kinases, two mammalian HRI-related protein kinases (Hri1 and Hri2) and the Gcn2 ortholog, regulate protein synthesis in response to cellular stress conditions. In this study, we demonstrate that both Hri1 and Hri2 exhibited an autokinase activity, specifically phosphorylated eIF2α, and functionally replaced the endogenous Saccharomyces cerevisiae Gcn2. We further show that Gcn2, but not Hri1 or Hri2, is activated early after exposure to hydrogen peroxide and methyl methanesulfonate (MMS). Cells lacking Gcn2 exhibit a later activation of Hri2. The activated MAPK Sty1 negatively regulates Gcn2 and Hri2 activities under oxidative stress but not in response to MMS. In contrast, Hri2 is the primary activated eIF2α kinase in response to heat shock. In this case, the activation of Sty1 appears to be transitory and does not contribute to the modulation of the eIF2α kinase stress pathway. In strains lacking Hri2, a type 2A protein phosphatase is activated soon after heat shock to reduce eIF2α phosphorylation. Finally, the MAPK Sty1, but not the eIF2α kinases, is essential for survival upon oxidative stress or heat shock, but not upon MMS treatment. These findings point to a regulatory coordination between the Sty1 MAPK and eIF2α kinase pathways for a particular range of stress responses. © 2010, American Society for Microbiology. | Descripción: | et al. | Versión del editor: | http://dx.doi.org/10.1128/EC.00185-09 | URI: | http://hdl.handle.net/10261/45875 | DOI: | 10.1128/EC.00185-09 | ISSN: | 1535-9778 |
| Aparece en las colecciones: | (IBMCC) Artículos (CBM) Artículos |
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