English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/45686
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Robust dynamical pattern formation from a multifunctional minimal genetic circuit

AuthorsRodrigo, Guillermo ; Carrera, Javier ; Elena, Santiago F. ; Elena, Santiago F. ; Jaramillo, Alfonso
Issue Date22-Apr-2010
PublisherBioMed Central
CitationBMC Systems Biology. 22;4(1):48 (2010)
AbstractAbstract Background A practical problem during the analysis of natural networks is their complexity, thus the use of synthetic circuits would allow to unveil the natural mechanisms of operation. Autocatalytic gene regulatory networks play an important role in shaping the development of multicellular organisms, whereas oscillatory circuits are used to control gene expression under variable environments such as the light-dark cycle. Results We propose a new mechanism to generate developmental patterns and oscillations using a minimal number of genes. For this, we design a synthetic gene circuit with an antagonistic self-regulation to study the spatio-temporal control of protein expression. Here, we show that our minimal system can behave as a biological clock or memory, and it exhibites an inherent robustness due to a quorum sensing mechanism. We analyze this property by accounting for molecular noise in an heterogeneous population. We also show how the period of the oscillations is tunable by environmental signals, and we study the bifurcations of the system by constructing different phase diagrams. Conclusions As this minimal circuit is based on a single transcriptional unit, it provides a new mechanism based on post-translational interactions to generate targeted spatio-temporal behavior.
Appears in Collections:(IBMCP) Artículos
Files in This Item:
File Description SizeFormat 
1752-0509-4-48.xml63,08 kBXMLView/Open
1752-0509-4-48.pdf1,09 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.