English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/45585
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Mechanisms of oocyte development in European sea bass (Dicentrarchus labrax L.): investigations via application of unilateral ovariectomy

AuthorsGarcía-López, Ángel ; Sánchez-Amaya, María Isabel ; Tyler, Charles R.; Prat, Francisco
KeywordsBass
Brain
Fish proteins
Oocytes
Ovariectomy
Pituitary gland
Testosterone
Issue Date24-May-2011
PublisherSociety for Reproduction and Fertility
CitationReproduction 142(2): 243-253 (2011)
AbstractUnilateral ovariectomy (ULO) was performed in European sea bass (Dicentrarchus labrax L.) during late pre-vitellogenesis/early vitellogenesis. Plasma steroid levels and the expression of a suite of potential oogenesis-relevant genes in the ovary, brain, and pituitary were evaluated with the aim of understanding their involvement in the compensatory oocyte development occurring within the remaining ovarian lobe. After 69 days of surgery the remaining ovarian lobe in ULO fish was gravimetrically equivalent to an intact-paired ovary of sham operated, control fish. This compensatory ovarian growth was based on an increased number of early perinucleolar oocytes and mid-late stage vitellogenic follicles without an apparent recruitment of primary oocytes into the secondary growth phase. Plasma steroid levels were similar in ULO and control females at all time points analyzed, suggesting an increased steroid production of the remaining ovarian lobe in hemi-castrated females. Results of the gene expression survey conducted indicate that the signaling pathways mediated by Fsh and Gnrh1 constitute the central axes orchestrating the observed ovarian compensatory growth. In addition, steroid receptors, Star protein, Igfs, and members of the transforming growth factor beta superfamily including anti-Mullerian hormone and bone morphogenetic protein 4 were identified as potentially relevant players within this process, although their specific actions and interactions remain to be established. Our results demonstrate that ULO provides an excellent in vivo model for elucidating the interconnected endocrine and molecular mechanisms controlling oocyte development in European sea bass.
Description11 páginas, 5 figuras.
Publisher version (URL)http://dx.doi.org/10.1530/REP-11-0040
URIhttp://hdl.handle.net/10261/45585
DOI10.1530/REP-11-0040
ISSN1470-1626
E-ISSN1741-7899
Appears in Collections:(ICMAN) Artículos
Files in This Item:
File Description SizeFormat 
mechanisms_oocyte_Garcia.pdf346,11 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.