Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/45449
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Rodríguez, Héctor | - |
dc.contributor.author | Rivas, Blanca de las | - |
dc.contributor.author | Muñoz, Rosario | - |
dc.contributor.author | Mancheño, Jose M. | - |
dc.date.accessioned | 2012-02-13T10:17:43Z | - |
dc.date.available | 2012-02-13T10:17:43Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Acta Crystallographica Section F Structural Biology and Crystallization Communications 63: 300–303 (2007) | es_ES |
dc.identifier.issn | 1744-3091 | - |
dc.identifier.uri | http://hdl.handle.net/10261/45449 | - |
dc.description.abstract | The substrate-inducible p-coumaric acid decarboxylase (PDC) from Lactobacillus plantarum has been overexpressed in Escherichia coli, purified and confirmed to possess decarboxylase activity. The recombinant His6-tagged enzyme was crystallized using the hanging-drop vapour-diffusion method from a solution containing 20%(w/v) PEG 4000, 12%(w/v) 2-propanol, 0.2 M sodium acetate, 0.1 M Tris–HCl pH 8.0 with 0.1 M barium chloride as an additive. Diffraction data were collected in-house to 2.04 A ° resolution. Crystals belonged to the tetragonal space group P43, with unit-cell parameters a = b = 43.15, c = 231.86 A ° . The estimated Matthews coefficient was 2.36 A ° 3 Da 1, corresponding to 48% solvent content, which is consistent with the presence of two protein molecules in the asymmetric unit. The structure of PDC has been determined by the molecular-replacement method. Currently, the structure of PDC complexed with substrate analogues is in progress, with the aim of elucidating the structural basis of the catalytic mechanism | es_ES |
dc.description.sponsorship | JMM thanks the Ministerio de Educación y Ciencia for a research grant (BFU2004-01554/BMC; DGCYT) in support of his research. This work was also supported by grants AGL2005-00470 (CICYT) and S-0505/AGR000153 (CAM). HR is a recipient of a I3P predoctoral fellowship from the CSIC. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | John Wiley & Sons | es_ES |
dc.relation.isversionof | Postprint | - |
dc.rights | openAccess | es_ES |
dc.subject | p-coumaric acid decarboxylase | es_ES |
dc.subject | Lactobacillus plantarum | es_ES |
dc.title | Overexpression, purification, crystallization and preliminary structural studies of p-coumaric acid decarboxylase from Lactobacillus plantarum | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1107/S1744309107008846 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1107/S1744309107008846 | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | - |
dc.contributor.funder | Dirección General de Investigación Científica y Técnica, DGICT (España) | - |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100008737 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.pmid | 17401200 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (IFI) Artículos (IQF) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
lactobacillus_plantarum_Rodriguez.pdf | 252,83 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
2
checked on 25-mar-2024
SCOPUSTM
Citations
14
checked on 15-abr-2024
WEB OF SCIENCETM
Citations
14
checked on 24-feb-2024
Page view(s)
325
checked on 20-abr-2024
Download(s)
164
checked on 20-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.