English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/45449
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Overexpression, purification, crystallization and preliminary structural studies of p-coumaric acid decarboxylase from Lactobacillus plantarum

AuthorsRodríguez, Héctor; Rivas, Blanca de las ; Muñoz, Rosario ; Mancheño, Jose M.
Keywordsp-coumaric acid decarboxylase
Lactobacillus plantarum
Issue Date2007
PublisherJohn Wiley & Sons
CitationActa Crystallographica Section F Structural Biology and Crystallization Communications 63: 300–303 (2007)
AbstractThe substrate-inducible p-coumaric acid decarboxylase (PDC) from Lactobacillus plantarum has been overexpressed in Escherichia coli, purified and confirmed to possess decarboxylase activity. The recombinant His6-tagged enzyme was crystallized using the hanging-drop vapour-diffusion method from a solution containing 20%(w/v) PEG 4000, 12%(w/v) 2-propanol, 0.2 M sodium acetate, 0.1 M Tris–HCl pH 8.0 with 0.1 M barium chloride as an additive. Diffraction data were collected in-house to 2.04 A ° resolution. Crystals belonged to the tetragonal space group P43, with unit-cell parameters a = b = 43.15, c = 231.86 A ° . The estimated Matthews coefficient was 2.36 A ° 3 Da 1, corresponding to 48% solvent content, which is consistent with the presence of two protein molecules in the asymmetric unit. The structure of PDC has been determined by the molecular-replacement method. Currently, the structure of PDC complexed with substrate analogues is in progress, with the aim of elucidating the structural basis of the catalytic mechanism
Publisher version (URL)http://dx.doi.org/10.1107/S1744309107008846
URIhttp://hdl.handle.net/10261/45449
DOI10.1107/S1744309107008846
ISSN1744-3091
Appears in Collections:(IFI) Artículos
(IQFR) Artículos
Files in This Item:
File Description SizeFormat 
lactobacillus_plantarum_Rodriguez.pdf252,83 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.