English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/45311
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

PCR detection of food bacteria producing the biogenic amines histamine, tyramine, putrescine, and cadaverine

AutorRivas, Blanca de las ; Marcobal, Ángela; Carrascosa, Alfonso V. ; Muñoz, Rosario
Fecha de publicación2006
EditorInternational Association for Food Protection
CitaciónJournal of Food Protection 69(10): 2509-2514 (2006)
ResumenThis study describes an easy PCR method for the detection of foodborne bacteria that potentially produce histamine, tyramine, putrescine, and cadaverine. Synthetic oligonucleotide pairs for the specific detection of the gene coding for each group of bacterial histidine, tyrosine, ornithine, or lysine decarboxylases were designed. Under the conditions used in this study, the assay yielded fragments of 372 and 531 bp from histidine decarboxylase-encoding genes, a 825-bp fragment from tyrosine decarboxylases, fragments of 624 and 1,440 bp from ornithine decarboxylases, and 1,098- and 1,185-bp fragments from lysine decarboxylases. This is the first PCR method for detection of cadaverine-producing bacteria. The method was successfully applied to several biogenic amine-producing bacterial strains
URIhttp://hdl.handle.net/10261/45311
ISSN0362-028X
Aparece en las colecciones: (IFI) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.