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Deletion of BCY1 from the Sacharomyces cerevisiae genome is semi-dominant and induces autolytic phenotypes suitable for improvement of sparkling wines

AuthorsTabera, Laura ; Muñoz, Rosario ; González García, Ramón
Issue DateApr-2006
PublisherAmerican Society for Microbiology
CitationApplied and environmental microbiology 72(4): 2351–2358 (2006)
AbstractAutolysis of Saccharomyces cerevisiae is the main source of molecules that contribute to the quality of sparkling wines made by the traditional method. In this work the possibility of accelerating this slow process in order to improve the quality of sparkling wines by using genetically engineered wine yeast strains was explored. The effect of partial or total deletion of BCY1 (which encodes a regulatory subunit of cAMPdependent protein kinase A) in haploid and diploid (heterozygous and homozygous) yeast strains was studied. We proved that heterozygous strains having partial or complete BCY1 deletions have a semidominant phenotype for several of the properties studied, including autolysis under simulated second-fermentation conditions, in contrast to previously published reports describing mutations in BCY1 as recessive. Considering the degree of autolysis, ethanol tolerance, and technical feasibility, we propose that deletion of the 3 end of the open reading frame of a single copy of BCY1 is a way to improve the quality of sparkling wines
Publisher version (URL)http://dx.doi.org/10.1128/AEM.72.4.2351-2358.2006
Appears in Collections:(IFI) Artículos
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