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Title

Identification of the ornithine decarboxylase gene in the putrescine-producer Oenococcus oeni BIFI-83

AuthorsMarcobal, Ángela; Rivas, Blanca de las ; Moreno-Arribas, M. Victoria ; Muñoz, Rosario
KeywordsDecarboxylase
Biogenic amines
Putrescine
Ornithine
Lactic acid bacteria
Oenococcus oeni
Issue Date2004
CitationFEMS Microbiology Letters 239: 213–220 (2004)
AbstractWe report here the identification of an ornithine decarboxylase (ODC) gene in the putrescine-producer Oenococcus oeni BIFI-83 strain. The gene contains a 2,235-nucleotide open reading frame encoding a 745-amino acid residues protein with a deduced molecular mass of 81 kDa. The primary structure of the ODC deduced from the nucleotide sequence has a consensus sequence containing the pyridoxal-5-phosphate (PLP) binding domain, and the critical amino acids residues involved in enzymatic activity are also conserved. As determined by BLAST analysis, the deduced amino acid sequence of the odc gene shares a 67% identity with the ODC protein from Lactobacillus 30a. The odc gene appears to be rarely present in the genome of O. oeni, since in a screening for the presence of this gene in 42 oenococcal strains none of the strains possessed an odc gene copy.
Publisher version (URL)http://dx.doi.org/10.1016/j.femsle.2004.08.036
URIhttp://hdl.handle.net/10261/44782
DOI10.1016/j.femsle.2004.08.036
ISSN0378-1097
Appears in Collections:(IFI) Artículos
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