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Título

Role of melanocortin receptor accessory proteins in the function of zebrafish melanocortin receptor type 2

AutorAgulleiro Gozalbo, Maria Josep ; Roy, Simon; Sánchez, Elisa ; Puchol, Sara; Gallo-Payet, Nicole; Cerdá-Reverter, José Miguel
Palabras claveACTH
MRAP
MC2R
HPA-axis
Stress
Fish
Fecha de publicaciónmay-2010
EditorElsevier
CitaciónMolecular and Cellular Endocrinology 320(1-2): 145-152 (2010)
ResumenIn this paper, we identify three different MRAPs in zebrafish, zfMRAP1, zfMRAP2a and zfMRAP2b, and demonstrate that zfMC2R is not functional in the absence of MRAP expression. ZfMRAP1 expression was restricted to adipose tissue and the anterior kidney whereas MRAP2a and MRAP2b were expressed in all the tissues tested. Quantification of surface receptor and immunofluorescence studies indicated that the receptor is unable to translocate to membrane in the absence of MRAP isoforms. MRAP1 and MRAP2b are localized in the plasma membrane in the absence of zfMC2R expression but MRAP2b is retained in perinuclear position. MRAP1 and MRAP2a displayed an equivalent translocation capacity to the membrane of zfMC2R but only zfMRAP1 expression led to intracellular cAMP increases after ACTH stimulation. ZfMRAP2b had no effect on zfMC2R activity but both zfMRAP2 isoforms enhanced the zfMRAP1-assited cAMP intracellular increase, suggesting an interaction between zfMRAP1 and zfMRAP2s when regulating zfMC2R activity.
Descripción19 p., figuras, tablas y bibliografía
Versión del editorhttp://dx.doi.org/10.1016/j.mce.2010.01.032
URIhttp://hdl.handle.net/10261/44603
DOI10.1016/j.mce.2010.01.032
ISSN0303-7207
E-ISSN1872-8057
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