English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/44391
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Altered Levels of Acetylcholinesterase in Alzheimer Plasma

AuthorsGarcía Ayllón, María Salud; Riba Llena, Iolanda; Serra Basante, Carol; Alom, Jordi; Boopathy, Rathnam; Sáez-Valero, Javier
Issue Date14-Jan-2010
PublisherPublic Library of Science
CitationPLoS ONE 5(1): e8701 (2010)
AbstractBackground Many studies have been conducted in an extensive effort to identify alterations in blood cholinesterase levels as a consequence of disease, including the analysis of acetylcholinesterase (AChE) in plasma. Conventional assays using selective cholinesterase inhibitors have not been particularly successful as excess amounts of butyrylcholinesterase (BuChE) pose a major problem. Principal Findings Here we have estimated the levels of AChE activity in human plasma by first immunoprecipitating BuChE and measuring AChE activity in the immunodepleted plasma. Human plasma AChE activity levels were ~20 nmol/min/mL, about 160 times lower than BuChE. The majority of AChE species are the light G1+G2 forms and not G4 tetramers. The levels and pattern of the molecular forms are similar to that observed in individuals with silent BuChE. We have also compared plasma AChE with the enzyme pattern obtained from human liver, red blood cells, cerebrospinal fluid (CSF) and brain, by sedimentation analysis, Western blotting and lectin-binding analysis. Finally, a selective increase of AChE activity was detected in plasma from Alzheimer's disease (AD) patients compared to age and gender-matched controls. This increase correlates with an increase in the G1+G2 forms, the subset of AChE species which are increased in Alzheimer's brain. Western blot analysis demonstrated that a 78 kDa immunoreactive AChE protein band was also increased in Alzheimer's plasma, attributed in part to AChE-T subunits common in brain and CSF. Conclusion Plasma AChE might have potential as an indicator of disease progress and prognosis in AD and warrants further investigation.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pone.0008701
URIhttp://hdl.handle.net/10261/44391
DOI10.1371/journal.pone.0008701
ISSN1932-6203
Appears in Collections:(IN) Artículos
Files in This Item:
File Description SizeFormat 
journal.pone.0008701.pdf495,64 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.