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dc.contributor.authorGuynet, Catherine-
dc.contributor.authorCuevas, Ana-
dc.contributor.authorMoncalián, Gabriel-
dc.contributor.authorCruz, Fernando de la-
dc.identifier.citationPLoS Genetics 7(5): e1002073 (2011)es_ES
dc.descriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License.-- Trabajo presentado al "4th Congresss of European Microbiologists - FEMS 2011".-
dc.description.abstractThe conjugative plasmid R388 and a number of other plasmids carry an operon, stbABC, adjacent to the origin of conjugative transfer. We investigated the role of the stbA, stbB, and stbC genes. Deletion of stbA affected both conjugation and stability. It led to a 50-fold increase in R388 transfer frequency, as well as to high plasmid loss. In contrast, deletion of stbB abolished conjugation but provoked no change in plasmid stability. Deletion of stbC showed no effect, neither in conjugation nor in stability. Deletion of the entire stb operon had no effect on conjugation, which remained as in the wild-type plasmid, but led to a plasmid loss phenotype similar to that of the R388ΔstbA mutant. We concluded that StbA is required for plasmid stability and that StbA and StbB control conjugation. We next observed the intracellular positioning of R388 DNA molecules and showed that they localize as discrete foci evenly distributed in live Escherichia coli cells. Plasmid instability of the R388ΔΔstbA mutant correlated with aberrant localization of the plasmid DNA molecules as clusters, either at one cell pole, at both poles, or at the cell center. In contrast, plasmid molecules in the R388ΔΔstbB mutant were mostly excluded from the cell poles. Thus, results indicate that defects in both plasmid maintenance and transfer are a consequence of variations in the intracellular positioning of plasmid DNA. We propose that StbA and StbB constitute an atypical plasmid stabilization system that reconciles two modes of plasmid R388 physiology: a maintenance mode (replication and segregation) and a propagation mode (conjugation). The consequences of this novel concept in plasmid physiology will be discussed.es_ES
dc.description.sponsorshipThis work was supported by grant BFU2008-00995/BMC from Ministerio de Ciencia e Innovación (MCINN, Spain), grant RD06/0008/1012 from Instituto de Salud Carlos III, and grant 248919/FP7-ICT-2009-4 from the European VII Framework Program. CG was the recipient of fellowships from Fondation pour la Recherche Médicale (SPE20080512320) and European Molecular Biology Organization (157-2008).es_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher’s version-
dc.titleThe stb operon balances the requirements for vegetative stability and conjugative transfer of plasmid R388es_ES
dc.description.peerreviewedPeer reviewedes_ES
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