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Characterization of flavins in roots of Fe-deficient strategy I plants, with a focus on Medicago truncatula

AuthorsRodríguez-Celma, Jorge ; Vázquez-Reina, Saúl ; Orduna, Jesús ; Abadía Bayona, Anunciación ; Abadía Bayona, Javier ; Álvarez-Fernández, Ana ; López-Millán, Ana Flor
KeywordsIron deficiency
Mass spectrometry
Medicago truncatula
Riboflavin derivative identification
Issue DateDec-2011
PublisherOxford University Press
CitationPlant and Cell Physiology 52(12): 2173-2189 (2011)
AbstractThe root accumulation and excretion of riboflavin (Rbfl) and Rbfl derivatives have been studied in the model legume species Medicago truncatula, grown in hydroponics in two different Fe deficiency conditions, with and without CaCO3. Using high resolution mass spectrometry techniques coupled to liquid chromatography, three different flavin derivatives not previously reported in plants, putatively identified as 7-hydroxy-Rbfl, 7a-hydroxy-Rbfl and 7-carboxy-Rbfl, were found along with Rbfl in Fe-deficient M. truncatula roots. In the presence of CaCO3 most of the flavins were accumulated in the roots, whereas in the absence of CaCO3 there was partial export to the nutrient solution. The major flavins in roots and nutrient solution were Rbfl and 7-hydroxy-Rbfl, respectively. Flavins were located in the root cortex and epidermal cells, preferentially in a root region near the apex that also exhibited increased ferric chelate reductase (FCR) activity. Six out of 15 different species of horticultural interest showed root increases in both Rbfl (four of them also having Rbfl derivatives) and FCR. No significant correlation was found between Rbfl and either phosphoenolpyruvate carboxylase or FCR activities, whereas the latter two showed a good correlation between them. The possible roles of Rbfl and Rbfl derivatives in roots and nutrient solutions are discussed. Medicago truncatula is proposed as a model system for flavin studies.
Description17 páginas, 7 figuras, 3 tablas.
Publisher version (URL)http://dx.doi.org/10.1093/pcp/pcr149
Appears in Collections:(EEAD) Artículos
(ICMA) Artículos
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