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Early Transcriptional Defense Responses in Arabidopsis Cell Suspension Culture under High-Light Conditions

AuthorsGonzález Pérez, Sergio CSIC; Gutiérrez Merino, Jorge; García-García, Francisco; Osuna, Daniel; Dopazo, Joaquín; Lorenzo, Óscar; Revuelta Doval, José Luis CSIC ORCID; Arellano, Juan B. CSIC ORCID CVN
KeywordsHigh light stress
Arabidopsis thaliana
Cell suspension culture
singlet oxygen
Early transcriptional analysis
Defense response
Hormone signalling pathway
Issue DateJul-2011
PublisherAmerican Society of Plant Biologists
CitationPlant Physiology 156:1439-1456
AbstractThe early transcriptional defense responses and reactive oxygen species (ROS) production in Arabidopsis (Arabidopsis thaliana) cell suspension culture (ACSC), containing functional chloroplasts, were examined at high light (HL). The transcriptional analysis revealed that most of the ROS markers identified among the 449 transcripts with significant differential expression were transcripts specifically up-regulated by singlet oxygen (1O2). On the contrary, minimal correlation was established with transcripts specifically up-regulated by superoxide radical or hydrogen peroxide. The transcriptional analysis was supported by fluorescence microscopy experiments. The incubation of ACSC with the 1O2 sensor green reagent and 2′,7′-dichlorofluorescein diacetate showed that the 30-min-HL-treated cultures emitted fluorescence that corresponded with the production of 1O2 but not of hydrogen peroxide. Furthermore, the in vivo photodamage of the D1 protein of photosystem II indicated that the photogeneration of 1O2 took place within the photosystem II reaction center. Functional enrichment analyses identified transcripts that are key components of the ROS signaling transduction pathway in plants as well as others encoding transcription factors that regulate both ROS scavenging and water deficit stress. A meta-analysis examining the transcriptional profiles of mutants and hormone treatments in Arabidopsis showed a high correlation between ACSC at HL and the fluorescent mutant family of Arabidopsis, a producer of 1O2 in plastids. Intriguingly, a high correlation was also observed with ABA deficient1 and more axillary growth4, two mutants with defects in the biosynthesis pathways of two key (apo)carotenoid-derived plant hormones (i.e. abscisic acid and strigolactones, respectively). ACSC has proven to be a valuable system for studying early transcriptional responses to HL stress.
Publisher version (URL)http://www.plantphysiol.org/content/156/3/1439.long#fn-1
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