English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/43703
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


An improved PCR method for endogenous DNA retrieval in contaminated Neandertal samples based on the use of blocking primers

AuthorsGigli, Elena ; Rasmussen, Morten; Civit, Sergi; Rosas, Antonio ; Rasilla, Marco de la; Fortea, Javier; Gilbert, M. Thomas P.; Willerslev, Eske; Lalueza-Fox, Carles
Blocking primers
Issue DateDec-2009
CitationJournal of Archaeological Science 36(12): 2676-2679 (2009)
AbstractNeandertal skeletal remains are usually contaminated with modern human DNA derived from handling and washing of the specimens during excavation. Despite the fact that the distinct Neandertal haplotypes allow the design of specific primer pairs, for instance in most of the mitochondrial DNA (mtDNA) hypervariable region 1 (HVR1), the human contaminants can often outnumber the endogenous DNA, thus preventing a successful retrieval of Neandertal sequences. We have developed a novel PCR method, based on the use of blocking primers that preferentially bind to modern human contaminant DNA and block their amplification, and greatly improve the efficiency of Neandertal DNA retrieval. We tested the method in four El Sidrón Neandertal samples (two teeth and two bone fragments) with different contamination levels and taphonomic conditions, and we have been able to significantly increase the Neandertal yield from figures around 25.23% (5–69.6%) up to 90.18% (75.3–100%).
Description4 páginas, 2 tablas.
Publisher version (URL)http://dx.doi.org/10.1016/j.jas.2009.08.004
Appears in Collections:(MNCN) Artículos
(IBE) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.