English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/43701
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Brownie, a Gene Involved in Building Complex Respiratory Devices in Insect Eggshells

AuthorsIrles, Paula; Bellés, Xavier ; Piulachs, Maria-Dolors
Issue DateDec-2009
PublisherPublic Library of Science
CitationPLoS ONE 4(12): e8353 (2009)
Abstract[Background]: Insect eggshells must combine protection for the yolk and embryo with provisions for respiration and for the entry of sperm, which are ensured by aeropyles and micropyles, respectively. Insects which oviposit the eggs in an egg-case have a double problem of respiration as gas exchange then involves two barriers. An example of this situation is found in the cockroach Blattella germanica, where the aeropyle and the micropyle are combined in a complex structure called the sponge-like body. The sponge-like body has been well described morphologically, but nothing is known about how it is built up. [Methodology/Principal Findings]: In a library designed to find genes expressed during late chorion formation in B. germanica, we isolated the novel sequence Bg30009 (now called Brownie), which was outstanding due to its high copy number. In the present work, we show that Brownie is expressed in the follicle cells localized in the anterior pole of the oocyte in late choriogenesis. RNA interference (RNAi) of Brownie impaired correct formation of the sponge-like body and, as a result, the egg-case was also ill-formed and the eggs were not viable. [Conclusions/Significance]: Results indicate that the novel gene Brownie plays a pivotal role in building up the sponge-like body. Brownie is the first reported gene involved in the construction of complex eggshell respiratory structures.
Description7 páginas, 4 figuras, 1 tabla.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pone.0008353
URIhttp://hdl.handle.net/10261/43701
DOI10.1371/journal.pone.0008353
ISSN1932-6203
Appears in Collections:(IBE) Artículos
Files in This Item:
File Description SizeFormat 
journal.pone.0008353[1].pdf977,34 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.