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Title

Mycosporine-like amino acid (MAAs) production by Heterocapasa sp. (Dinophyceae) in indoor cultures

AuthorsMontero, Olimpio; Lubián, Luis M.
KeywordsHeterocapsa sp.
Mycosporine-like amino acids
MAAs
HPLC
Ultraviolet radiation
Issue Date27-May-2003
PublisherElsevier
CitationBiomolecular Engineering 20(4-6): 183-189 (2003)
AbstractThe possibility of using mycosporine-like amino acids (MAAs), with an apparent sunscreen function in nature, as ultraviolet radiation (UVR) blockers to prevent skin injury has been raised by diverse authors. Production of MAAs by the dinoflagellate Heterocapsa sp. (Dinophyceae) is shown here. Three major peaks with absorption maxima at 330.8, 332.0 and 333.2 nm were detected by high performance liquid chromatography (HPLC) analysis of methanolic extracts in all tested conditions. Analysis of crude extract by mass spectroscopy with electrospray ionization (MS-EI) showed a set of molecular ions ([M+H]+) with main peaks being at m/z 242.4, 288.4, 303.3 and 333.3 u.m.a. According to these data, along with retention times, the MAA profile of Heterocapsa sp. is assumed to be composed of shinorine (λmax=334 nm), mycosporine–2-glycine (λmax=331 nm) and palythinol (λmax=332 nm). A constitutive MAA content of about 4 μg (106 cells)−1 was measured under exposure to PAR only. A maximal accumulation of MAA per culture volume of 1.1 mg l−1 was obtained after 72 h of exposure to PAR+UVA, while the highest production rate (0.025 mg l−1 h−1) was computed after 24 h of exposure to PAR+UVA+UVB.
Description7 páginas, 8 figuras, 1 tabla.
Publisher version (URL)http://dx.doi.org/10.1016/S1389-0344(03)00050-9
URIhttp://hdl.handle.net/10261/43386
DOI10.1016/S1389-0344(03)00050-9
ISSN1389-0344
Appears in Collections:(ICMAN) Artículos
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