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Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes

Other TitlesMultiplex screening of the human Y chromosome using TaqMan probes
AuthorsMartínez-Cruz, Begoña; Ziegle, Janet; Sanz, Paula; Sotelo, Graciela; Anglada, Roger; Plaza, Stéphanie; Comas, David
Issue Date31-May-2011
PublisherBioMed Central
CitationInvestigative Genetics 2: 13 (2011)
Abstract[Background] The analysis of human Y-chromosome variation in the context of population genetics and forensics requires the genotyping of dozens to hundreds of selected single-nucleotide polymorphisms (SNPs). In the present study, we developed a 121-plex (121 SNPs in a single array) TaqMan array capable of distinguishing most haplogroups and subhaplogroups on the Y-chromosome human phylogeny in Europe.
[Results] We present data from 264 samples from several European areas and ethnic groups. The array developed in this study shows >99% accuracy of assignation to the Y human phylogeny (with an average call rate of genotypes >96%).
[Conclusions] We have created and evaluated a robust and accurate Y-chromosome multiplex which minimises the possible errors due to mixup when typing the same sample in several independent reactions.
DescriptionGrupo de trabajo: The Genographic Consortium.
Publisher version (URL)http://dx.doi.org/10.1186/2041-2223-2-13
Appears in Collections:(IBE) Artículos
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