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Development of a method for quantitative analysis of the major whey proteins by capillary electrophoresis with on-capillary derivatization and laser-induced fluorescence detection

AuthorsVeledo, MaríaTeresa; Frutos, Mercedes de; Diez-Masa, Jose Carlos
KeywordsOn-capillary derivatization
Capillary electrophoresis
Laser-induced fluorescence
Whey proteins
Issue Date2005
PublisherJohn Wiley & Sons
CitationJournal of Separation Science 28 (9-10) : 935-940 (2005)
AbstractThe main whey proteins have been derivatized on-capillary with 3-(2-furoyl)quinoline- 2-carboxaldehyde (FQ) and analyzed using a laboratory-made capillary electrophore- sis apparatus provided with a laser-induced fluorescence detector. Several param- eters controlling on-capillary derivatization of proteins, including pH, mixing time, reaction time, concentration of the reagents (potassium cyanide and FQ), and reac- tion temperature, were optimized. Coefficient variations were lower than 1% for migration time and 7% for peak height. Assay detection limits for the different proteins were in the range 5 nM to 10 nM. The method developed was applied to the separa- tion of the major whey proteins in a laboratory-made cheese whey and in an infant food formulated with milk. In addition, the b-LG content of these samples was quanti- tated. The results showed good agreement with those given by an RP-HPLC method and with those reported in the literature.
Publisher version (URL)http://dx.doi.org/10.1002/jssc.200500016
Appears in Collections:(IQOG) Artículos
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