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Title

CZE of human alpha-1-acid glycoprotein for qualitative and quantitative comparison of samples from different pathological conditions

AuthorsLacunza, Izaskun ; Sanz Perucha, Jesús ; Diez-Masa, Jose Carlos; Frutos, Mercedes de
KeywordsAlpha-1-acid glycoprotein
CE
Disease marker forms
Glycoprotein
Issue Date2006
PublisherJohn Wiley & Sons
CitationElectrophoresis 27 : 4205–4214 (2006)
AbstractAlpha-1-acid glycoprotein (AGP) presents different forms, which may arise from dif- ferences in the amino acid sequence and/or in the glycosidic part of the protein. Changes in forms of AGP have been described in literature as a possible tumor marker. While most previous works have approached the study of glycopeptides and/or gly- cans obtained after fragmentation of the protein, in this work, a CZE method is devel- oped to separate up to eleven peaks of intact forms of AGP. A computer program developed in our laboratory is used to select the migration parameters that make possible an accurate assignment of AGP peaks. Electropherograms of AGP samples purified from sera of cancer patients and healthy donors are qualitatively and quanti- tatively compared. Percentages of correct assignment of AGP peaks close to 100% are achieved by using either the migration time of each peak relative to that of the EOF marker or the effective electrophoretic mobility of the peaks. The computer program permits to select, among different hypotheses for peak allotment, that one providing the highest accuracy of assignment. In this way, some peaks with different charge-to-mass ratio and a different distribution of area percentage of AGP forms are observed when comparing samples from sick and healthy individuals. Thus, a method that permits to compare AGP forms existing in sera of individuals with different pathophysiological situations has been developed. A potential for using AGP forms analyzed by CZE as a disease marker and for using this technique for screening purposes is envisaged.
Publisher version (URL)http://dx.doi.org/10.1002/elps.200600304
URIhttp://hdl.handle.net/10261/43324
DOI10.1002/elps.200600304
ISSN0173-0835
Appears in Collections:(IQOG) Artículos
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