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Título

Immunocytohistochemical characterization of pituitary cells of the bluefin tuna, Thunnus thynnus L

AutorRodríguez-Gómez, F. J.; Rendón-Unceta, M. C.; Piñuela, C.; Muñoz-Cueto, José Antonio; Jiménez-Tenorio, Natalia; Sarasquete, Carmen
Palabras claveAdenohypophysis
Hormones
Pituitary cells
Immunocytohistochemistry
Tuna
Teleost
Fecha de publicación2001
EditorUniversidad de Murcia
CitaciónHistology and Histopathology (Cellular and Molecular Biology ) 16(2): 443-451 (2001)
ResumenIn this paper we report the first complete mapping of the pituitary in a tuna species. The various different adenohypophysis cell types of the bluefin tuna, Thunnus thynnus L. have been identified and located using different antisera against mammalian and piscine hormones and various histochemical techniques: PAS, Alcian Blue pH 2.5 and lectins -ConA and WGA- (Neutral and Acidic Glycoproteins); Bromophenol Blue (Proteins) and Tioglycollate-Ferric-Ferricianide-Fe111 (-S-S- groups). Prolactin (PRL) and adrenocorticotrophic (ACTH) cells were located in the rostral pars distalis (RPD) of the pituitary, while the proximal pars distalis (PPD) displayed gonadotrophic (GTH), thyrotrophic (TSH), somatotrophic (GH) and also a few PRL cells. Moreover, somatolactin (SL) and melanotrophic (MSH) cells were identified inside the pars intermedia (PI). Interestingly, some SL-immunoreactive fibers were dso detected in the neurohypophysis. Some GTH cells were also located on the exterior surface of the PI. Glycoproteins containing mannose (Man) andlor glucose (Glc); N-acetyl-glucosamine (GlcNAc) andlor sialic acid sugar residues, as well as -S-S- groups, were observed in GTH, TSH and SL cells. The Bromophenol Blue technique stained amphiphilic SL, acidophilic GH cells and weakly ACTH cells. GH and ACTH cells were unreactive to PAS, Alcian Blue, Tioglycollate-Ferric- Ferricianide-Fe111 and lectin (Con A and WGA) techniques. Finally, PAS reaction was positive in amphiphilic SL cells, which were PbH unreactive, while MSH and ACTH cells were stained with PbH technique.
Descripción9 páginas, 2 figuras, 1 tabla.
Versión del editorhttp://revistas.um.es/hh/article/view/124291
URIhttp://hdl.handle.net/10261/43296
ISSN0213-3911
E-ISSN1699-5848
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