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Título

In vivo genotoxicity and stress defences in three flatfish species exposed to CuSO4

AutorChairi, H.; Fernández-Díaz, C. ; Navas, J. I.; Manchado, Manuel; Rebordinos, Laureana; Blasco, Julián
Palabras claveGenotoxicity
Flatfish
CuSO4
Antioxidant enzymes
Stress protein
Comet assay
Fecha de publicación3-ago-2010
EditorElsevier
CitaciónEcotoxicology and Environmental Safety 73(6): 1279-1285 (2010)
ResumenWe have used the comet assay to analyse, after 3 h, 24 h and 6 days, the genotoxic effect in vivo of applying a single intraperitoneal injection of CuSO4, at a concentration of 2 mg/kg, to adult specimens of Solea senegalensis, Dicologlossa cuneata and Scophthalmus rhombus. Metals content (Cu, Zn and Cd) in liver was also measured. The activity of key stress defences was evaluated by analysing antioxidant enzyme activity (catalase (CAT), superoxide dismutase (SOD), total glutathione peroxidase (t-GPX), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH)), metallothionein (MT) and heat shock proteins (HSP70 and HSP60). The results show that CuSO4 intake generates high and cumulative levels of genotoxicity throughout the 6 days in all 3 species. After 6 days, metals content detected in specimens showed significant differences from controls. Inter-species differences were detected in enzyme activity (P<0.05). A clear response to CuSO4 was detected only in S. rhombus, with an increase of MT and a decrease of HSPs. Variations in antioxidant defence levels and their comparative responses to the stress-inducing agent are discussed.
Descripción7 páginas, 4 figuras, 1 tabla.
Versión del editorhttp://dx.doi.org/10.1016/j.ecoenv.2010.07.028
URIhttp://hdl.handle.net/10261/43234
DOI10.1016/j.ecoenv.2010.07.028
ISSN0147-6513
E-ISSN1090-2414
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