Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/42893
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Effect of haemolysis and repeated freeze-thawing cycles on wild boar serum antibody testing by ELISA |
Autor: | Boadella, Mariana CSIC ORCID; Gortázar, Christian CSIC ORCID | Fecha de publicación: | 16-nov-2011 | Editor: | BioMed Central | Citación: | BMC Research Notes 4: 498 (2012) | Resumen: | [Background]: Monitoring wildlife diseases is needed to identify changes in disease occurrence. Wildlife blood samples are valuable for this purpose but are often gathered haemolysed. To maximise information, sera often go through repeated analysis and freeze-thaw cycles. Herein, we used samples of clean and haemolysed Eurasian wild boar (Sus scrofa) serum stored at -20°C and thawed up to five times to study the effects of both treatments on the outcome of a commercial ELISA test for the detection of antibodies against Suid Herpesvirus 1 (ADV). [Results]: The estimated prevalence of antibodies against ADV was 50-53% for clean and haemolysed sera. Hence, haemolysis did not reduce the mean observed serum antibody prevalence. However, 10 samples changed their classification after repeated freeze-thawing. This included 3 (15%) of the clean sera and 7 (41%) of the haemolysed sera. [Conclusions]: We recommend (1) establishing more restrictive cut-off values when testing wildlife sera, (2) recording serum quality prior to sample banking, (3) recording the number of freezing-thawing cycles and (4) store sera in various aliquots to reduce repeated usage. For instance, sera with more than 3 freeze-thaw cycles and a haemolysis of over 3 on a scale of 4 should better be discarded for serum antibody monitoring. Even clean (almost not haemolysed) sera should not go through more than 5 freeze-thaw cycles. | Versión del editor: | http://dx.doi.org/10.1186/1756-0500-4-498 | URI: | http://hdl.handle.net/10261/42893 | DOI: | 10.1186/1756-0500-4-498 | ISSN: | 1756-0500 |
Aparece en las colecciones: | (IREC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
1756-0500-4-498.xml | 42,91 kB | XML | Visualizar/Abrir | |
1756-0500-4-498.pdf | 255,65 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
16
checked on 26-mar-2024
SCOPUSTM
Citations
36
checked on 11-abr-2024
Page view(s)
323
checked on 18-abr-2024
Download(s)
388
checked on 18-abr-2024