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Beta-Adrenoceptor and GRK3 expression in human lymphocytes is related to blood pressure and urinary albumin excretion

Other TitlesBeta-adrenoceptors and GRK3 in hypertension
AuthorsOliver, Eduardo; Rovira, Eduardo; Montó, Fermí; Valldecabres, Carmen; Julve, Raúl; Muedra, Vicente; Ruiz, Nuria; Barettino, Domingo ; D’Ocon, Pilar
Urinary albumin excretion
ACE inhibitors
Issue DateJun-2010
PublisherLippincott Williams & Wilkins
CitationJournal of hypertension 28(6): 1281-9 (2010)
AbstractOBJECTIVE: The objective of our work was to analyze if changes in the expression of betaadrenoceptors (b-ARs) and G-protein-coupled receptor kinases (GRKs) in human lymphocytes - a practical surrogate for myocardial or vascular cells - are related to the hypertensive state and its clinical consequences. METHODS: real time quantitative RT-PCR was employed to evaluate the expression of the three b-ARs (b1, b2, b3) and three GRKs (GRK2, GRK3, GRK5) in human lymphocytes obtained from both normotensive and hypertensive subjects, some of whom had been treated with blockers of the renin-angiotensin system. Office blood pressure, 24-hour ambulatory blood pressure, urinary albumin excretion and serum biochemical profile were also recorded. RESULTS AND CONCLUSIONS: b1-AR expression levels were higher in circulating lymphocytes from hypertensive patients (2-ΔΔCt =2.135 ± 0.4252*, vs control group), but this difference was not observed when these subjects were treated with blockers of the renin-angiotensin system. b1- AR levels directly correlated (r2=0.5711, P=0.0185) with urinary albumin excretion in microalbuminuric patients, which relates alterations of this receptor to cardiovascular risk. An inverse correlation was observed between the expression levels of b2-AR and diastolic blood pressure (r2=0.2078, P=0.0031), suggesting that b2-ARlevels in lymphocytes mirror their expression in vascular cells, in which b2-AR-mediated relaxation regulates vascular resistance. mrRNA levels for GRK3 were inversely correlated with systolic and diastolic blood presure (day, night and 24h), which suggests a protective role for GRK3 in the regulation of human blood pressure, as supported by previous findings in transgenic mice.
Description31 p., figuras, abreviaturas y bibliografía
Publisher version (URL)http://dx.doi.org/10.1097/HJH.0b013e3283383564
Appears in Collections:(IBV) Artículos
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