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Title

Isolation and characterization of nine polymorphic microsatellite markers in the two-banded bream (Diplodus vulgaris) and cross-species amplification in the white bream (Diplodus sargus) and the saddled bream (Oblada melanura).

AuthorsRoques, Séverine ; Galarza, Juan A.; Macpherson, Enrique ; Turner, George F.; Carreras-Carbonell, Josep ; Rico, Ciro
KeywordsDiplodus
Microsatellite
Oblada melanura
Sea bream
Sparidae
Issue Date2007
PublisherBlackwell Publishing
CitationMolecular Ecology Notes 7: 661-663 (2007)
AbstractWe have developed nine new microsatellite markers for the two-banded sea bream ( Diplodus vulgaris ) from an enriched genome library protocol. All these loci are polymorphic, with mean allelic diversity of 13 (range 5–21), and expected and observed heterozygosities from 0.641 to 0.932 and 0.428 to 0.914, respectively. Cross-species tests in two close-related species of the genus Diplodus ( D. sargus and O. melanura ) revealed successful amplifications at 8 out of 9 loci, with mean allele number of 4.75 (range 2–8) and 5.50 (range 3–10), respectively. These results are consistent with the close phylogenetic relationships between the three species, indicating this set of primers might prove useful for studying the levels of genetic diversity and population differentiation in these three species and in other phylogenetically close species of the genus Diplodus and Sparus .
Description3 páginas, 2 tablas.
Publisher version (URL)http://dx.doi.org/10.1111/j.1471-8286.2006.01667.x
URIhttp://hdl.handle.net/10261/42123
DOI10.1111/j.1471-8286.2006.01667.x
ISSN1755-098X
Appears in Collections:(CEAB) Artículos
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