Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/4174
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Autoradiographic distribution of 5-HT7 receptors in the human brain using [3H]mesulergine: comparison to other mammalian species

AuthorsMartín Cora, Francisco Javier; Pazos, Ángel CSIC ORCID
Keywords5-hydroxytryptamine
5-HT7 receptor
Central nervous system
Human
Guinea-pig
Rat
Quantitative autoradiography
Issue Date7-Jan-2004
PublisherNature Publishing Group
British Pharmacological Society
CitationBr J Pharmacol. 141(1): 92-104 (2004).
http://dx.doi.org/10.1038/sj.bjp.0705576
Abstract[Objective] The main aim of this investigation was to delineate the distribution of the 5-HT7 receptor in human brain. Autoradiographic studies in guinea-pig and rat brain were also carried out in order to revisit and compare the anatomical distribution of 5-HT7 receptors in different mammalian species.
[Methods] Binding studies were performed in rat frontal cortex membranes using 10 nM [3H]mesulergine in the presence of raclopride (10 μM) and DOI (0.8 μM). Under these conditions, a binding site with pharmacological characteristics consistent with those of the 5-HT7 receptors was identified (rank order of binding affinity values: 5-CT > 5-HT > 5-MeOT > mesulergine ≈methiothepin >8-OH-DPAT = spiperone ≈(+)-butaclamol >> imipramine ≈(±)-pindolol >> ondansetron ≈clonidine ≈prazosin).
[Results] The autoradiographic studies revealed that the anatomical distribution of 5-HT7 receptors throughout the human brain was heterogenous. High densities were found over the caudate and putamen nuclei, the pyramidal layer of the CA2 field of the hippocampus, the centromedial thalamic nucleus, and the dorsal raphe nucleus. The inner layer of the frontal cortex, the dentate gyrus of the hippocampus, the subthalamic nucleus and superior colliculus, among others, presented intermediate concentrations of 5-HT7 receptors. A similar brain anatomical distribution of 5-HT7 receptors was observed in all three mammalian species studied.
[Conclusions] By using [3H]mesulergine, we have mapped for the first time the anatomical distribution of 5-HT7 receptors in the human brain, overcoming the limitations previously found in radiometric studies with other radioligands, and also revisiting the distribution in guinea-pig and rat brain.
DescriptionPMCID: PMC1574165.-- Final full text version available at: http://dx.doi.org/10.1038/sj.bjp.0705576
URIhttp://hdl.handle.net/10261/4174
DOI10.1038/sj.bjp.0705576
ISSN0007-1188
Appears in Collections:(IBBTEC) Artículos

Show full item record
Review this work

PubMed Central
Citations

17
checked on May 20, 2022

SCOPUSTM   
Citations

93
checked on May 18, 2022

WEB OF SCIENCETM
Citations

89
checked on May 20, 2022

Page view(s)

347
checked on May 22, 2022

Google ScholarTM

Check

Altmetric

Dimensions


Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.