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Influence of carbon source and concentration on the in vitro development of olive (Olea europaea L.) zygotic embryos and explants raised from them

AuthorsGarcía Fernández, José Luis ; Troncoso Mendoza, Javier ; Sarmiento Solís, Rafael ; Troncoso de Arce, Antonio
KeywordsOlea europaea L.
Bud explants
Embryo germination
In vitro culture
Issue Date2002
CitationPlant Cell, Tissue and Organ Culture 69(1): 95-100 (2002)
AbstractThe influence of sucrose or mannitol on in vitro zygotic embryo germination, seedling development and explant propagation of olive tree (Olea europaea L.) was compared. Embryos germinated without sucrose in the medium but for adequate development of the seedlings to yield viable plants, a carbohydrate supply was necessary; both sucrose and mannitol were equally suitable for this purpose. However, when explants obtained from in vitro germinated embryos were cultured with mannitol or sucrose, then the polyalcohol promoted significantly more growth than sucrose by increasing shoot length, pairs of leaves formed, and breaking apical dominance. This improved the in vitro culture of olive plant material, thus allowing new olive clonal lines to be obtained in shorter times. This will assist in future breeding experiments with the species.
Description6 pages, 5 tables, 33 references.
Publisher version (URL)http://dx.doi.org/10.1023/A:1015086104389
Appears in Collections:(IRNAS) Artículos
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