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Título: | Molecular and biochemical characterization of the tetralin degradation pathway in Rhodococcus sp. strain TFB |
Autor: | Tomás-Gallardo, Laura CSIC ORCID ; Santero, Eduardo CSIC ORCID; Camafeita, Emilio; Calvo, Enrique; Schlömann, Michael; Floriano Pardal, Belén CSIC ORCID | Palabras clave: | Bacterial proteins Base sequence Biodegradation Electrophoresis Metabolic networks and pathways Protomer regions Rhodococcus Tetrahydronaphthalenes |
Fecha de publicación: | 18-feb-2009 | Editor: | Society for Applied Microbiology Blackwell Publishing |
Citación: | Microbial Biotechnology 2(2): 262-273 (2009) | Resumen: | The tetralin biodegradation pathway in Rhodococcus sp. strain TFB, a Gram-positive bacterium resistant to genetic manipulation, was characterized using a proteomic approach. Relative protein expression in cell free extracts from tetralin- and glucose-grown cells was compared using the 2D-DIGE technique. Identification of proteins specifically expressed in tetralin-grown cells was used to characterize a complete set of genes involved in tetralin degradation by reverse genetics. We propose a tetralin degradation pathway analogous to that described for Sphingomonas macrogolitabida strain TFA. TFB thn genes are organized into three operons; two contain all of the structural genes and are transcribed in the same direction, while the third operon, thnST, is transcribed in the opposite direction and encodes a two-component regulatory system, whose transcription is higher in tetralin-grown cells. In addition to tetralin induction, TFB thn structural genes are subject to glucose repression. Primer extension assays and translational thnA1::gfp and thnS::gfp fusions were used to characterize putative promoter regions. A mutational analysis of the thnA1 promoter region allowed us to define nucleotides within the cis regulatory elements that are important for the control of thn gene expression. | Descripción: | 12 páginas, figuras. | Versión del editor: | http://dx.doi.org/10.1111/j.1751-7915.2009.00086.x | URI: | http://hdl.handle.net/10261/41615 | DOI: | 10.1111/j.1751-7915.2009.00086.x | ISSN: | 1751-7907 | E-ISSN: | 1751-7915 |
Aparece en las colecciones: | (CABD) Artículos |
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