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Título

Bicarbonate binding to the water-oxidizing complex in the photosystem II. A Fourier transform infrared spectroscopy study

Autor Yruela Guerrero, Inmaculada ; Allakhverdiev, Suleyman I.; Ibarra, José V.; Klimov, Vyacheslav V.
Palabras clave Photosystem II
Bicarbonate
Donor side
Fourier transform
Infrared spectroscopy
Fecha de publicación abr-1998
EditorElsevier
Citación FEBS Lett 425 (3): 396-400, 1998
ResumenThe light-induced Fourier transform infrared difference (FT-IR) spectrum originating from the donor side of O2-evolving photosystem (PS) II was obtained in non-depleted and CO2-depleted PSII membrane preparations. The observed spectrum free of contributions from the acceptor side signals was achieved by employing 2 mM/18 mM ferri-/ferrocyanide as a redox couple. This spectrum showed main positive bands at 1589 and 1365 cm−1 and negative bands at 1560, 1541, 1522 and 1507 cm−1. CO-depleted PSII preparations showed a quite different spectrum. The main positive and negative bands disappeared after depletion of bicarbonate. The addition of bicarbonate partially restored those bands again. Comparison between difference FT-IR spectra of untreated and bicarbonate-depleted PSII membranes indicated that the positive bands at 1589 and 1365 cm−1 can be assigned to COO− stretching modes from bicarbonate. The higher frequency corresponds to uas (COO−) and the lower frequency to us (COO−). 13C-Labeling FT-IR measurements confirmed these findings and also suggested that the negative band at 1560 cm−1 can be ascribed to uas (COO−). The data are discussed in the framework of the suggestion that bicarbonate can be a ligand to the Mn-containing water-oxidizing complex of PSII.
Descripción The definitive version is available at http://www.febsletters.org/article/S0014-5793(98)00271-3/
URI http://hdl.handle.net/10261/4148
DOI10.1016/S0014-5793(98)00271-3
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