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dc.contributor.authorMontes, Tamara-
dc.contributor.authorGrazú, Valeria-
dc.contributor.authorManso, Isabel-
dc.contributor.authorGalán, Beatriz-
dc.contributor.authorLópez-Gallego, Fernando-
dc.contributor.authorGonzález García, Ramón-
dc.contributor.authorHermoso, Juan A.-
dc.contributor.authorGarcía, José Luis-
dc.contributor.authorGuisán, José Manuel-
dc.contributor.authorFernández-Lafuente, Roberto-
dc.date.accessioned2011-10-18T12:05:58Z-
dc.date.available2011-10-18T12:05:58Z-
dc.date.issued2007-01-08-
dc.identifier.citationAdvanced Synthesis & Catalysis 349(3): 459–464 (2007)es_ES
dc.identifier.issn1615-4150-
dc.identifier.urihttp://hdl.handle.net/10261/41321-
dc.description.abstractPenicillin G acylase (PGA) is an enzyme that hardly interacts with polycationic polymers (e.g., polyethyleneimine, PEI) and thus the enzyme cannot be stabilized against the action of organic solvents by its co-immobilization with the polymer in the same support, neither covalently attached to the support nor adsorbed on the already immobilized enzyme. However, a new mutant PGA bearing eight additional Glu residues homogenously distributed throughout the enzyme surface may interact with the polymer. The co-immobilization of the enzyme and PEI on glyoxyl-agarose allows one to fully take advantage of the stabilization produced by the multipoint covalent attachment and by the protective hydrophilic micro-environment generated by the polycationic polymer, enabling a significant stabilization of the immobilized PGA in the presence of organic solvents.es_ES
dc.description.sponsorshipThe authors gratefully recognize the support from the Spanish CICYT (Projects BIO-2005–8576 and BIO-2003–05309- C04–02).es_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.rightsclosedAccesses_ES
dc.subjectEnzyme stabilityes_ES
dc.subjectMicro-environmentses_ES
dc.subjectOrganic solventses_ES
dc.subjectPolyethyleneiminees_ES
dc.titleImproved Stabilization of Genetically Modified Penicillin G Acylase in the Presence of Organic Cosolvents by Co- Immobilization of the Enzyme with Polyethyleneiminees_ES
dc.typeartículoes_ES
dc.identifier.doi10.1002/adsc.200600313-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1002/adsc.200600313es_ES
dc.identifier.e-issn1615-4169-
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