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Title

Analysis of trace amounts of bovine b-lactoglobulin in infant formulas by capillary electrophoresis with on-capillary derivatization and laser-induced fluorescence detection

AuthorsVeledo, Maria Teresa; Frutos, Mercedes de; Diez-Masa, Jose Carlos
KeywordsOn-capillary derivatization
Capillary electrophoresis
Laser-induced fluorescence
b-Lactoglobulin
Hypoallergenic formula
Infant formulas
Allergen
LIF
Quantitation
Issue Date2005
PublisherJohn Wiley & Sons
CitationJournal of Separation Science 28 (9-10) : 941-947(2005)
AbstractBovine b-lactoglobulin (bLG) has been described by several authors as the main allergen present in cow's milk. It can induce allergic reactions even at the low concen- tration existing in hypoallergenic formulas based on hydrolyzed cow’s milk proteins (generally lower than lM). In this paper, the usefulness of a capillary electrophoresis method with on-capillary derivatization and laser-induced fluorescence detection for the analysis of trace amounts of bLG in a commercial hypoallergenic formula has been demonstrated. To confirm the identity of the peak of bLG based on migration time, an immunorecognition step employing an anti-bLG antibody was performed. bLG was quantitated in the whey and casein fractions of the hypoallergenic formula. The concentration of bLG in the whey fraction of the formula was about 3 orders of magnitude lower than the average value present in cow’s milk. In the casein fraction of the formula, the concentration of bLG was about 1 order of magnitude lower than in the whey fraction. The method developed was also used for the quality control of three cereal-based infant foods formulated without milk to test the presence or absence of bLG as an indicator of milk contamination during the fabrication process. bLG in a concentration of 10 –7 M or higher was not observed in any of the cereal- based infant formulas analyzed.
Publisher version (URL)http://dx.doi.org/10.1002/jssc.200500020
URIhttp://hdl.handle.net/10261/41298
DOI10.1002/jssc.200500020
ISSN1615-9306
Appears in Collections:(IQOG) Artículos
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