English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/41097
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Distinct roles for NtrC and GlnK in nitrogen regulation of the Pseudomonas sp. strain ADP cyanuric acid utilization operon

AuthorsGarcía-González, Vicente ; Jiménez-Fernández, Alicia ; Hervás, Ana B. ; Canosa, Inés ; Santero, Eduardo ; Govantes, Fernando
KeywordsNitrogen control
PII proteins
Atrazine degradation
Pseudomonas
Issue Date4-Sep-2009
PublisherFederation of European Microbiological Societies
Blackwell Publishing
CitationFEMS Microbiology Letters 300(2): 222-229 (2009)
AbstractThe Pseudomonas sp. strain ADP atzDEF operon encodes the enzymes involved in cyanuric acid mineralization, the final stage of the s-triazine herbicide atrazine degradative pathway. We have previously shown that atzDEF is under nitrogen control in both its natural host and Pseudomonas putida KT2442. Expression of atzDEF requires the divergently encoded LysR-type transcriptional regulator AtzR. Here, we take advantage of the poor induction of atzDEF in Escherichia coli to identify Pseudomonas factors involved in nitrogen control of atzDEF expression. Simultaneous production of P. putida NtrC and GlnK, along with AtzR, restored the normal atzDEF regulatory pattern. Gene expression analysis in E. coli and P. putida indicated that NtrC activates atzR expression, while the role of GlnK is to promote AtzR activation of atzDEF under nitrogen limitation. Activation of atzDEF in a mutant background deficient in GlnK uridylylation suggests that post-translational modification is not strictly required for transduction of the nitrogen limitation signal to AtzR. The present data and our previous results are integrated in a regulatory circuit that describes all the known responses of the atzDEF operon.
Description8 páginas, 1 figura, 3 tablas.
Publisher version (URL)http://dx.doi.org/10.1111/j.1574-6968.2009.01784.x
URIhttp://hdl.handle.net/10261/41097
DOI10.1111/j.1574-6968.2009.01784.x
ISSN0378-1097
E-ISSN1574-6968
Appears in Collections:(CABD) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.