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A new methodology to obtain wine yeast strains overproducing mannoproteins

AuthorsQuirós Asensio, Manuel ; González Ramos, Daniel; Tabera, Laura ; González García, Ramón
KeywordsWine yeast
UV mutagenesis
Williopsis saturnus
Issue Date30-Apr-2010
CitationInternational Journal of Food Microbiology 139(1-2): 9-14 (2010)
AbstractYeast mannoproteins are highly glycosylated proteins that are covalently bound to the β-1,3-glucan present in the yeast cell wall. Among their outstanding enological properties, yeast mannoproteins contribute to several aspects of wine quality by protecting against protein haze, reducing astringency, retaining aroma compounds and stimulating growth of lactic-acid bacteria. The development of a non-recombinant method to obtain enological yeast strains overproducing mannoproteins would therefore be very useful. Our previous experience on the genetic determinants of the release of these molecules by Saccharomyces cerevisiae has allowed us to propose a new methodology to isolate and characterize wine yeast that overproduce mannoproteins. The described methodology is based on the resistance of the killer 9 toxin produced by Williopsis saturnus, a feature linked to an altered biogenesis of the yeast cell wall.
Publisher version (URL)http://dx.doi.org/10.1016/j.ijfoodmicro.2010.02.014
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(IFI) Artículos
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